人源幼虫巨大致死性基因在非小细胞肺癌中的表达及意义

Reduction of Hugl-1 Gene Expression in NSCLC:Expression and Correlation with Clinicopathologic Parameters.

目的研究人源幼虫巨大致死性基因Hugl-1表达与人肺癌临床病理生理特征的关系,探讨Hugl-1基因表达与肺癌肿瘤细胞发生、侵袭的相关性。方法对50例NSCLC标本(含癌旁组织)和10例肺良性病变组织应用单克隆抗体、免疫组化SP法检测Hugl-1基因的蛋白表达情况。结果(1)肺癌肿瘤组织中Hugl-1基因表达水平显著低于癌旁组织和肺良性病变组织(P<0.05)。(2)肺癌组织中Hugl-1基因表达水平降低与肿瘤分期、淋巴结转移程度存在相关性(P<0.05),与肺癌组织学类型、细胞分化程度以及患者的性别、年龄无关。结论Hugl-1基因表达下降与肺癌的发生、发展过程有关。

Objective The aim of this study was to assess the expression of protein products of Hugl - 1 （human lethal - giant - lavae 1 ） in non - small cell lung cancer（ NSCLC）, to estimate their possible expression and to correlate the results of immunobistocbemical analysis with various clinicopatbologic parameters. Methods The expression of Hugl - 1 gene was detected by immunobistocbemical S - P method in 50 cases of NSCLC tissues ,50 para - cancer tissues and 10 cases of lung benign lesion tissues as control. Results （ 1 ） The expression level of Hugl - 1 gene was found to be significantly lower in NSCLC tissues than in para - cancer tissues and lung benign lesion tissues（ P 〈 0.05）. （2）The expression level of Hugl- 1 gene was closely related to PTNM stages and lymph node involvement in Lung cancer patients（ P 〈 0.05 ）. Conclusions Maintenance of Hugl - 1 gene may effect cell polarity, changes of epithelial polarity causes byperplasia and reduction of Hugl - 1 gene expression may be related to the patbogenesis and development of NSCLC.