趋化因子IL-8与胃癌组织中浸润炎性细胞相关性分析

Relationship of Chemokine Interleukin-8 mRNA Expression and Tumor Infiltrating Lymphocyte and Neutrophil in Gastric Carcinoma.

目的本文旨在探讨趋化因子IL-8与肿瘤浸润淋巴细胞、中性粒细胞在胃癌组织中的相关性,初步研究趋化因子IL-8对局部免疫力的影响及其在胃癌中的可能作用机制。方法采用针对T3、T4、T8的单克隆抗体,用SABC法对肿瘤组织切片进行免疫组织化学染色,然后对阳性细胞进行计数,组织中的中性粒细胞采用测定组织中的髓过氧化物酶的含量,来反映中性粒细胞的数目及其活性,用半定量反转录聚合酶链反应(RT-PCR)方法测定IL-8mRNA的表达。结果IL-8的基因表达水平与肿瘤中浸润的淋巴细胞及中性粒细胞均无显著相关性(P>0.05),但IL-8的表达与T4/T8的比值呈明显的负相关(γ=-0.52,P<0.05)。结论胃癌组织中趋化因子IL-8的表达增强,并不能特异性趋化T淋巴细胞及中性粒细胞,但与T4/T8相关,表明IL-8可能对局部免疫力有一定抑制作用,控制IL-8的表达,可能对提高局部免疫力有一定作用。

Objective To investigate the correlation of chemokine interleukin - 8 （ IL - 8 ） and tumor infiltrating lymphocyte, neutrophil in gastric cancer. Study the effect of chemokine IL - 8 on local region immunocompetence and the mechanism of IL - 8 in gastric cancer. Methods The distribution and quantitation of the subpopulations of tumor infiltrating lymphocyte including T3 ,T4 ,T8 and neutrophil were studied with SABC immunhistochemistry and myeloperoxidase （MPO） kits respectively. Results Interleukin -8 and T4/T8 has a negative correlations （ Y = - 0.52, P 〈 0.05 ） , Not like gastritis, IL - 8 has no relationship with the activity of MPO in gastric cancer. Conclusion Interleukin- 8 ,which is overexpressed in gastric cancer,doesn＇t attract T3 ,T4 ,T8 and neutrophil, but has a negative correlation with T4/TS, It Suggests IL -8 maybe suppress the local immunocompetence, so it maybe important to improe local immun reaction through down - regulate the expression of IL - 8.