孕早期超声辐照对胎鼠神经组织的影响

Effect on Nervous Tissue and Anaphase Diagnostic Ultrasound in Early Pregnancy.

目的孕鼠早期经诊断级超声辐照后,通过对出生前胎鼠神经组织的细胞生物学性状检查,以期进一步评价孕早期超声诊断的安全性。方法采用细胞培养,形态学方法观察初生鼠神经组织细胞的生长状况;通过细胞增生生长曲线检测及细胞周期时相的流式细胞仪检测来研究超声对胎儿神经组织细胞的影响。结果①形态学观察发现实验组与对照组的神经组织细胞生长状态没有明显的改变,均存在有少量的分裂相;②细胞增生生长曲线,实验组和对照组均有缓慢生长期,增生的高峰期及生长衰退期;③通过流式细胞检测,结果显示实验组与对照组各时相也无显著性差异。结论孕早期超声辐照一定时间对初生鼠脑组织的细胞形态,细胞生长状况及周期时相没有显著性改变。

Objective To assess the diagnosis safety by detecting the biological character of prenatal fetus rats＇ nervous tissue exposed to diagnostic ultrasound during earlier period. Methods Cell culture, morphology examining, cell proliferation curve measurement and flow cytometry detection were adopted. Results （1）After morphology examining, there are no difference between the treated group and control group. （2） We can see from the growth curve, the two groups has identical growth tendency. （3）Through flow cytometry detection, results show that there are no significance between two groups. Conclusion Diagnostic ultrasound in early pregnancy have no significant effect on prenatal fetus rats＇ nervous tissue.