应用自制组织芯片研究抑癌基因PTEN在口腔鳞癌中的表达

Expression of Tumor Suppressor Gene PTEN in Oral Squamous Cell Cancer Using Tissue Microarray Techniques

目的从蛋白水平探讨口腔鳞癌组织中肿瘤抑制基因PTEN的表达及临床病理意义。方法应用SP免疫组化染色法和组织芯片技术,检测10例正常口腔粘膜、10例口腔上皮单纯增生、15例口腔粘膜白斑及72例OSCC(其中高分化30例、中分化26例、低分化16例)组织中PTEN蛋白的表达,同时分析PTEN的表达与患者临床病理资料的关系。结果PTEN基因在口腔鳞癌、口腔粘膜白斑、口腔上皮单纯增生和正常口腔粘膜组织中的阳性率分别为72.2%(52/72)、93.3%(14/15)、100%(10/10)和100%(10/10),口腔鳞癌组与其他各组间有显著性差异(P<0.05);PTEN的阳性表达率在不同性别、年龄和TNM分期等临床病理参数间无显著性差异(P>0.05),但与淋巴结转移、组织分化程度等临床病理参数间有显著性差异(P<0.05)。结论PTEN基因表达的下调在OSCC的发生、发展中起着重要作用,其表达的异常可作为判断预后的参考指标之一。

Objective To explore expression of PTEN in oral squamous cell carcinoma （OSCC） and its clinicopathological significance Methods PTEN protein expression in paraffin embedded tissues from 10 cases of normal oral mucosa, 10 cases of oral epithelial hyperplasia, 15 cases of oral leukoplakia and 72 cases of OSCC was analyzed by SP immunohistochemical method and tissue microarray techniques, meanwhile analysed clinical pathological data. Results The positive rate of PTEN protein was 72. 2% （52/ 72） .93. 3% （14/15）. 100% （10/10） and 100% （10/10） in OSCC, oral leukoplakia, oral epithelial hyperplasia and normal oral mucosa, there was significant correlation among them（P〈0. 05）. PTEN expression was not obviously correlated with age, different gender, and TNM classification etc（P〉0. 05）, but there was significant correlation with nodal metastases and degree of OSCC （P〈0. 05）. Conclusion Loss of PTEN protein expression may play an important role on the tumorigenesis and development of OSCC, PTEN can be used as a reference marker for judging prognosis of patients with OSCC.