脆性组氨酸三联体基因及p53基因在肺癌组织中的表达及意义

Expression of Fragile Histidine Triay Gene(FHIT) and p53 in Lung Carcinoma and Its Significance

目的探讨脆性组氨酸三联体基因(FHIT)在肺癌组织中的表达及其与肺癌分化程度、组织学分类、p53表达的关系。方法利用组织芯片,运用免疫组织化学技术,对110例肺癌及25例良性病变的肺组织标本分别进行FHIT、p53蛋白检测。结果①良性病变肺组织FHIT异常表达率为16%,而肺癌组织中FHIT异常表达率为85.5%。肺癌组织与良性病变肺组织相比,差异有极显著性(P<0.01);分化程度不同肺癌组织,FHIT表达差异有极显著性(P<0.01),且FHIT基因表达与肺癌组织分化程度正相关(P<0.01);组织学分类不同的肺癌组织,FHIT基因表达差异无统计学意义(P>0.05);②p53阴性表达组和阳性表达组,FHIT的表达差异无统计学意义(P>0.05);p53异常表达率为53.6%,FHIT异常表达率为85.4%,两者比较差异有极显著性(P<0.01)。p53异常表达与FHIT异常表达共同检出率为93.6%。结论①FHIT蛋白可能成为肺癌早期诊断的更有效分子标记物,p53蛋白与FHIT蛋白的联合检测有助于提高肺癌的检出率;②FHIT蛋白影响肺肿瘤细胞的分化方向,为临床药物开发提供新思路。

Objective To investigate the expression of FHIT gene in lung carcinoma and its relationship with differentiation degree and histological types and the expression of p53 gene. Methods The expressions of FHIT and p53 were detected by immunohistochemistry method and tissue microarray technique in the specimens of 110 lung carcinoma and 25 benign lung lesions. Results （1）Abnormal expression rate of FHIT was 16% in benign lung lesions, 85. 5% in lung carcinoma, the difference was very significant between them（P〈0. 01 ）; when the differentiation degree was different, the difference of FHIT expression in the lung carcinoma was very significant（P〈0. 01 ）, and FHIT expression was positive correlation with differentiation degree（r = 0. 608, P〈0. 01 ） ; when the histological types were different, the difference of FHIT expression in the lung carcinoma was not statistical significance（P〈0. 05） ;（2）comparing the negative expression group with the positive one of p53, the difference of FHIT expression in the lung carcinoma was not statistical significance（P〉0. 05） ;in lung carcinoma abnormal expression rate of p53 was 53. 6%, abnormal expression rate of FHIT was 85. 4%, the difference was statistical significance（P 〈0. 01 ） ;the rate of union detection was 93. 6 %. Conclusion （3）FHIT protein may become the molecular maker of earlier period diagnosing of lung carcinoma, and the union detection of FHIT, p53 protein will help to improve the detection rate; QFHIT protein influences direction of differentiation of the lung carcinoma cell, and this will offer new thinking for the development of clinical tumour drug.