SDF-1对内皮祖细胞增殖迁移的影响及AMD3100对其的干预

Effect of AMD3100 on SDF-1 induced proliferation and migration of endothelial progenitor cells

目的探讨基质细胞衍生因子-1(stromal cell derived factor-1,SDF-1)对小鼠骨髓源性内皮祖细胞(endotheli-al progenitor cells,EPCs)增殖迁移的影响及AMD3100对其的干预效应。方法分别用不同浓度的SDF-1α刺激EPCs,观察SDF-1α的促增殖效应再用不同浓度的AMD3100干预5ng/ml SDF-1α的促增殖效应及单用AMD3100与EPCs孵育,观察AMD3100对EPCs增殖的影响。MTT比色法检测EPCs的增殖情况。用1、10ng/ml SDF-1α诱导EPCs迁移,或先用10ng/ml AMD3100与EPCs孵育,再做SDF-1α诱导的/无SDF-1α诱导的迁移实验。用改良的Boyden小室测定EPCs迁移能力。结果SDF-1α剂量依赖性增强EPCs增殖能力,10ng/ml AMD3100即完全阻断5ng/ml SDF-1α的促进EPCs增殖效应,单用AMD3100孵育可抑制EPCs增殖,与阴性对照组比较差异显著(P<0·01);单用AMD3100孵育抑制EPCs迁移,与阴性对照组比较差异显著(P<0·01)。结论SDF-1α可增强EPCs增殖迁移能力,AMD3100可完全阻断其促增殖和诱导迁移效应,单用AMD3100抑制EPCs增殖迁移。

Objective To investigate the effect of stromal cell derived factor-1 （ SDF-1 ） on the proliferation and migration of endothelial progenitor ceils （EPCs） and whether such an effect could be blocked with AMD3100. Methods EPCs in 96-well plates were co-cultured with SDF-1α （ 1,5, 10 ng/ml）, or AMD3100 or 5 ng/ml SDF-1α ＋ AMD3100 （ 10, 50, 100 ng/ml） for 24 h. EPCs proliferation was assayed with MTr. EPCs was incubated with or without 10 ng/ml AMD3100 for 1 h, then induced by SDF-1α or without to start migration at 37℃ for 6 h. EPCs migration was evaluated by using a modified Boyden chamber assay. Results SDF-1 α increased EPCs proliferation activity in a dose-dependent manner, but its effect induced by 5 ng/ml SDF-1α was inhibited completely with 10 ng/ml AMD3100. The proliferation of EPCs treated with AMD3100 alone was inhibited. SDF-1α increased EPCs migration, but this effect was completely blocked by AMD3100. The migration of EPCs was reduced by AMD3100 alone. Conclusion SDF-1α can improve EPCs proliferation and migration, and its effect could be blocked with AMD3100. The treatment of AMD3100 alone decreases the proliferation and migration of EPCs.