过热与脂多糖复合应激促发和加重大鼠全身炎症反应综合征的研究

Combination of hyperthermia and LPS may advance and augment systemic inflammatory response syndrome in rats

目的探讨高温与脂多糖(LPS)复合应激大鼠血浆TNF-α、IL-6以及氧自由基MDA含量、SOD活力等指标的变化规律。方法将雄性SPF级Wistar大鼠分为:常温生理盐水组(C组)、高温生理盐水组(H组)、常温LPS组(L组)、高温LPS组(HL组)。HL、L组动物经尾静脉注射LPS10mg/kg(浓度为1mg/ml),H、C组动物经尾静脉注射0.9%NaCl10ml/kg。检测动物应激0、40、80、120min时血浆TNF-α、IL-6、MDA含量及SOD活力的变化。结果①HL组于应激40min时血浆TNF-α、IL-6水平即显著升高,TNF-α峰值(80min)显著高于其他各组TNF-α水平,IL-6表达水平极度升高,显著高于同时相各组。②应激40min时HL组动物血浆MDA水平显著升高,SOD活力水平显著下降,与同时相各组比较差异有统计学意义(P<0·05)。结论高温与LPS复合应激可能促发、扩大全身炎症反应综合征。

Objective To investigate the effects of co-exposure to LPS and heat on plasma tumor necrosis factor-α （ TNF-α）, interleukin-6 （ IL-6 ）, malondialdehyde （MDA） and activity of superoxide dismutase （SOD） in rats. Methods Male pathogen-free Wistar rats were randomly assigned to the following groups ： saline-injected normothermic control （Group C）, saline-injected heat exposure （Group H）, LPS-injected normothermic control （Group L）, LPS-injected heat exposure （Group HL）. Mean arterial pressure （MAP） was continually monitored. Plasma levels of TNF-α, IL-6, MDA and SOD were determined at 0, 40, 80, 120 min. Results The rats in Group HL displayed much lower MAP, higher MDA and lower SOD than the other 3 groups. The rats in Group HL displayed an early rise in plasma TNF-α, IL-6 at 40 min, the peak values of both at 80 min significantly higher than the other three groups at the same time piont. Conclusion Co-exposure to LPS and heat primes the rats to advance and augment systemic inflammatory response syndrome.