人子宫内膜细胞原代培养方法的改良

Improvement of primary culture methods for human endometrial cells

目的寻求一种简便高效的人子宫内膜上皮细胞和间质细胞分离、纯化与鉴定方法,建立稳定的子宫内膜细胞体外培养体系。方法简化Ryan等的子宫内膜细胞原代培养方法的取材,分离和培养条件,分离出高纯度的子宫内膜上皮细胞和间质细胞进行体外培养和传代。结果培养成功的子宫内膜上皮细胞与间质细胞均可稳定传代,体外生长10～50d,纯度均达90%以上。结论该方法简便高效,可建立稳定的人子宫内膜细胞体外培养体系。

Objective To explore the methods to isolate, purify and identify human endometrial cells, establish the stable endometrial cells thelial cells and stromal cells were and culture ocnditions. Results culture system in vitro. Methods Highly purified human endometrial epiobtained by improved Ryan＇ s procedure for specimen preparation, isolation The endometrial epithelial cells and strumal cells could be and survived 10 -50 days in vitro, and their purity was above 90%. Conclusion The improved primary cul- ture methods for human endometrial cells is convenient and of high performance. The stable human endometrial cells culture system in vitro is established, providing cell platform for studying the physiologic and pathologic mechanism of human endometrium and the drug action mechanism on human endometrium.