巨噬细胞集落刺激因子对肺癌细胞株A549在体外的抑制作用

Inhibitory effect of macrophage colony stimulating factor on lung cancer cell line A549

目的观察巨噬细胞集落刺激因子(macrophage colony stimulating factor,M-CSF)对肺腺癌细胞株A549的作用。方法观察肺腺癌A549细胞形态的变化。M-CSF对体外培养肺腺癌A549细胞的生长抑制作用采用MTT检测法,用流式细胞仪检测细胞DNA含量,分析M-CSF对细胞周期的影响。采用RT-PCR检测M-CSF受体mRNA表达的变化。结果M-CSF能显著抑制肺腺癌A549细胞的生长,并且呈浓度、时间依赖关系,在M-CSF浓度为10ng/ml时有最大抑制效应。流式细胞仪检测分析显示M-CSF作用后G0/G1期细胞增加。RT-PCR显示经M-CSF作用后M-CSF受体的mRNA表达水平有所下降。结论M-CSF对肺癌细胞株A549在体外有抑制作用。

Objective To explore the effect of macrophage colony stimulating factor （M-CSF） on lung cancer cell line A549. Methods After M-CSF at 0.1, 1 and 10 ng/ml was given to cultured A549 cells for 0, 24, 48, 72 and 92 h respectively, their morphological changes were observed with inverted microscopy, proliferation was measured by MTT assay, and cell cycles were determined by flow cytometry. RT-PCR was used to detect the change of expression of M-CSF receptor. Result The growth of A549 was significantly inhibited by M-CSF in a concentration- and time- dependent manner. The maximum response was obtained at 10 ng/ml of M-CSF. Flow cytometric analysis revealed that the treated A549 cells arrested at the Go/Gi phase of the cell cycle. RT-PCR showed that the mRNA expression of M-CSF receptor was reduced after the M-CSF treatment. Conclusion M-CSF has an anti-tumour activity on lung cancer cell A549.