人参皂甙Rg1对镉诱导的大鼠睾丸损伤的保护作用

Ginsenoside Rg1 protect against Cadmium-induced tesicular damage in rat

目的:探讨人参皂甙Rg1对镉诱导的大鼠睾丸损伤的保护作用及作用机制。方法:30只雄性wistar大鼠随机分为对照组、氯化镉组和Rg1+氯化镉组。氯化镉组大鼠按0.5mg(/kg·d)氯化镉腹腔染毒,Rg1+氯化镉组大鼠在0.5mg(/kg·d)氯化镉腹腔染毒2h前进行按100mg(/kg·d)Rg1灌胃处理,连续14天后将动物处死,分离附睾,测定精子数量和质量。分离睾丸,测定金属硫蛋白(MT)含量,TUNEL法检测睾丸切片凋亡情况。结果:氯化镉引起附睾中精子数量减少,质量降低,金属硫蛋白表达增加,睾丸中精细胞凋亡指数增加,而Rg1可以改善附睾中精子质量和数量,减少精细胞凋亡指数,并进一步上调金属硫蛋白表达。结论:人参皂甙Rg1对镉诱导的大鼠睾丸损伤具有保护作用,机制可能与上调金属硫蛋白表达有关。

bjective：To investigate the protective effect and the possible mechanism of Rg1 on Cadmium-induced tesicular damage in rat.Methods ：30 wistar rats were divided into 3 groups：the control group,the Cd group and the Cd＋Rg1 group.The Cd group rats injected intraperitoneally with 0.5 mg/（kg·d） cadmium for 14 days.The rats in the Cd＋Rg1 group were pretreated with 75mg/ （kg·d） Rg1 by intragastric administration before injected intraperitoneally with 0.5 mg/ （kg·d） cadmium.All animals were killed after 14 days,the sperm count,the movable sperm percentage and the abnormal sperm rate in epididymis were analyzed.The content of MT in testis were detected and the apoptosis of testis genital cells were assessed by TdT-mediated dUTP-hiotin nick end labeling （TUNEL）technique.Results：14 days after exposed to Cd caused a significant decrease in the sperm count and the movable sperm percentage.On the other hand,the abnormal sperm rate,the content of MT and the apoptosis percent of genital cells in testis were increased significantly.In the Cd＋Rg1 group,the sperm count,the movable sperm percentage and the content of MT were higher than that of Cd group,while the abnormal sperm rate and the apoptosis percent of genital cells in testis were significantly lower than that of Cd group.Conclusion：Rg1 have protective effect on on tesicular damage induced hy Cd possihly through upregulating the expression of MT in testis.