结肠癌中有丝分裂关卡基因hsMAD2表达的分析

Analysis of expression of mitosis checkpoint gene hsMAD2 in colorectal cancer

目的:研究结肠癌组织中有丝分裂关卡基因HsMAD2的表达。方法:采用定量real-timeRT-PCR方法检测18例结肠癌及正常结肠组织中hsMAD2基因表达水平。结果:以hsMAD2基因mRNA拷贝数/GAPDH基因mRNA拷贝数比值为hs-MAD2基因mRNA水平的相对定量值。结肠癌和正常组织中的hsMAD2基因mRNA拷贝数/GAPDH基因mRNA拷贝数比值分别为0.1235±0.0752和0.0312±0.0598。hsMAD2基因的表达水平在结肠癌中显著高于正常结肠组织。结论:hsMAD2基因在结肠癌组织中的高表达,可能与癌细胞的异常增殖有关,是结肠癌发生发展的原因,可为临床结肠癌的治疗提供有效的靶点。

Objective：To study the expression of mitosis checkpoint gene hsMAD2 in colorectal cancer. Methods：The expression of hsMAD2 gene mRNA in colorectal cancer and normal colorectal tissue of 18 cases was quantitatively detected by RT-PCR, Results：The ratio of hsMAD2 mRNA/ GAPDH mRNA in colorectal cancer and normal colorectal tissue were 0.1235 ±0.0752 and 0.0312±0.0598,respectively. The results suggested that the expression of hsMAD2 mRNA in colorectal cancer were significant higher than in normal colorectal tissue（P〈0.05）. Conchtsion：Compared with normal colorectal tissue,the expression of hsMAD2 mRNA in colorectal cancer was markedly increased. This probably indicated that to up-regulate the expression of hsMAD2 mRNA in colorectal cancer may be a very effective to abnormal proliferation of colorectal cancer cells. And hsMAD2 gene as a related gene to colorectal cancer can play an important role in colorectal cancer pathogenesis.