Expression and its clinical significance of HLA-G in HCMV-infected placenta] villi at early pregnant stage 被引量：1

Expression and its clinical significance of HLA-G in HCMV-infected placenta] villi at early pregnant stage

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摘要 Objective：To study the expression and its clinical significance of HLA-G in HCMV intrauterine infected placental villi at early pregnant stage. Methods：PCR （polymerase chain reaction） was used to screen the peripheral blood for HCMV-DNA in 462 women who had willingly undergone induced abortion. Then immunohistochemistry was also used to detect expressions of mouse anti-HCMV early antigen （HCMV-EA） and mouse anti-HLA-G in HCMV-DNA positive cases＇ placental villi. The difference of HLA-G expressions between the intrauterine infection group（HCMV-EA positives）, the intrauterine infection-free group（HCMV-EA negatives） and the normal control group （50 cases of healthy early placental villi） was compared. Results： Of the 78 cases, which were detected HCMV-DNA positive, 11 （14.10%） were HCMV-EA positive. Compared with the other two groups, HLA-G expressions in the intrauterine infection group were both obviously decreased（both P〈0. 001）. HLA-G expression positions in all three groups were mainly located in the cytotrophoblast. Conclusion：Intrauterine HCMV infection at early pregnant stage is closely related to HLA-G expression at the maternal-fetal interface. The virogenetic products may affect the expression of HLA-G at the maternal-fetal interface and that of its immunological function, thus leading to different clinical outcomes. Objective:To study the expression and its clinical significance of HLA-G in HCMV intrauterine infected placental villi at early pregnant stage. Methods:PCR (polymerase chain reaction) was used to screen the peripheral blood for HCMV-DNA in 462 women who had willingly undergone induced abortion. Then immunohistochemistry was also used to detect expressions of mouse anti-HCMV early antigen (HCMV-EA) and mouse anti-HLA-G in HCMV-DNA positive cases' placental villi. The difference of HLA-G expressions between the intrauterine infection group (HCMV-EA positives), the intrauterine infection-free group(HCMV-EA negatives) and the normal control group (SO cases of healthy early placental villi) was compared. Results: Of the 78 cases, which were detected HCMV-DNA positive, 11 (14. 10%) were HCMV-EA positive. Compared with the other two groups, HLA-G expressions in the intrauterine infection group were both obviously decreased (both P<0. 001). HLA-G expression positions in all three groups were mainly located in the cytotrophoblast. Conclusion:Intrauterine HCMV infection at early pregnant stage is closely related to HLA-G expression at the maternal-fetal interface. The virogenetic products may affect the expression of HLA-G at the maternal-fetal interface and that of its immunological function, thus leading to different clinical outcomes.

作者张欣文李芬盛秋于学文任永惠李学成

机构地区 Maternal and Child Health Center

出处《Journal of Medical Colleges of PLA(China)》 CAS 2007年第1期31-35,共5页 中国人民解放军军医大学学报（英文版）

基金 Surpported by a grant from the National Natural Science Foundation of China (No. 30170981)

关键词 human cytomegalovirus intrauterine infection HLA-G maternal-fetal interface 妊娠早期胎盘绒毛 HLA-G 巨细胞病毒感染子宫内感染基因表达

分类号 R714.25 [医药卫生—妇产科学]

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1[1]Fisher S,Genbacev O,Maidji E et al.Human cytomegalovirus infection of placental cytotroblasts in vitro and in utero:implications for transmission and pathogenesis[J].J Virol,2000;74:6808.
2[2]Boyoun P,Hokyung O,Sungwook L et al.The MHC class I homolog of human cytomegalovirus is resistant to down-regulation mediated by the unique short region protein (US)2,US3,US6,and US11 gene products[J].J Immunol,2002;168:3464.
3[4]Agrawal S,Pandey MK.The potential role of HLA-G polymorphism in maternal tolerance to the developing fetus[J].J Hematother Stem Cell Res,2003;12:749.
4[5]Gonen-Gross T,Achdout H,Gazit R et al.Complexes of HLAG protein on the cell surface are important for leukocyte Ig-like receptor-1 function[J].J Immunol,2003;171:1343.
5[6]Pizzato N,Garmy Susini B,Le Bouteiller P et al.Differential down-modulation of HLA-G and HLA A2 or -A3 cell surface expression following human cytomegalovirus infection[J].J Reprod Immunol,2004;62:3.
6[7]Terauchi M,Koi H,Hayano C et al.Placental extravillous cytotrophoblasts persistently express class I major histocompatibility complex molecules after human cytomegalovirus infection[J].J Virol,2003;77:8187.
7[8]Jason LP,Chantey M,Joyce S.Virus evasion of MHC class Ⅰ molecule presentation[J].J Immunol,2003;171:4473.
8[9]Cerboni C,Achour A,Warnmark A et al.Spontaneous mutations in the human CMV HLA class I homologue UL18 affect its binding to the inhibitory receptor LIR-1/ILT2/CD85j[J].Eur J Immunol,2006;36:732.