小鼠卵巢促性腺激素受体的免疫组化定位

Immunohistochemical Localization of Gonadotrophin Receptors in the Mouse Ovary

目的探明小鼠两种促性腺激素受体(FSHr、LHr)在卵巢的位置分布,揭示促性腺激素(GTH)调节卵巢机制及与卵泡发育分化的关系。方法运用免疫组化ABC法对小鼠卵巢FSHr、LHr分别进行定位染色,结合图像分析系统处理分析阳性切片。结果①FSHr阳性物质主要见于GC、TC、卵母细胞及间质细胞。随卵泡的发育,FSHr、LHr阳性细胞数量呈增长趋势,卵泡早期与中期之间阳性细胞数量差异显著,平均吸光度变化差异不显著。②LHr阳性物质主要见于卵泡TC、间质细胞、GC、卵母细胞,阳性物质着色以卵泡中、晚期较强,阳性细胞数量以卵泡中期与晚期之间差异显著。平均吸光度变化差异不显著。结论卵泡颗粒细胞、膜细胞上受体是接受促性腺激素的主要调节部位,受体数量与卵泡大小和发育程度有一定的正相关。

Objective To study the distribution and localization of FSH receptor （FSHr） and LH receptor （LHr） in the mouse ovary, and to elucidate the regulatory mechanism of GTH and the relationship between follicular development and expression of those receptors. Methods The localization of FSHr and LHr in mouse ovary were determined by immunohistochemical ABC techniques, analyzing the positive sections by an image analysis system. Results ① FSHr-immunoreactive substance was mainly distributed over granular cells, theca cells, oocytes and ovary interstitial cells. With the development of follicles, the quantity of the FSHr-immunoreactive and LHr-immunoreactive cells presented an increasing tendency. There were significant differences between early and intermediate follicular phases, but the change of the mean optical density was quite similar. ② LHr-immunoreactive substance was mainly distributed over theca cells, ovarian interstitial cells, granular cells and oocytes. The staining intensity of LHr-immunoreactive substance in late and intermediate follicular phases was stronger than that in early follicular phase. There was a significant difference between intermediate and late follicular phases. The change of the mean optical density was not significantly different. Conclusion The receptors on granular and theca cells were the main regulatory sites of GTH. There is a positive correlation of the quantity of receptors with follicle size and developmental degrees.