SIVmac251不同途径感染恒河猴急性期实验研究

Comparative Study on Acute Manifestation of Rhesus Monkeys Infected with SIVmac251 by Different Routes

目的建立SIV黏膜感染的恒河猴模型,并与静脉感染相比较,研究不同途径感染后病程进展是否存在差异。方法用SIVmac251经静脉、阴道、直肠3种途径分别感染恒河猴,定期采血,进行全血病毒分离,测定血浆病毒载量、CD4+/CD8+比值及抗体检测。结果感染后7d至目前56d为止,外周血单核淋巴细胞中前病毒DNA检测、全血病毒分离全部呈现阳性,只有直肠感染猴S172在7d和42d时出现较高病毒滴度;血浆病毒载量检测阳性出现先后顺序为静脉感染、直肠感染、阴道感染,14d达到高峰,然后迅速下降,变化趋势基本保持一致。血浆抗体检测从第14天开始3种途径感染的恒河猴体内抗体均为阳性;感染后CD4+/CD8+比值下降,在第28天时出现比例倒置。结论经不同途径均成功感染恒河猴后在病毒分离和血浆病毒载量等方面表现出了一定的差异。

Objective To establish a rhesus monkey model of SIV infection＇by mucosal route, and compare possible differences in disease course after SIV inoculation in rhesus monkeys by intravenous, intravaginal and intrarectal routes. Methods SIVmac251 strain was used to inoculate three rhesus monkeys by intravenous, intravaginal and intrarectal routes, respectively. Regular sampling, viral load detection, CD4^＋ cells measurement and virus isolation were approached to identify the results. Results From 7d to 56d （at present） after inoculation, the results of virus isolation, DNA-PCR detection were positive. Viral loads of plasma from S153, S172 and S142 were measured during earlier stage. The antibodies in plasma were detected positively from 14d and up to now. Ratio of CD4^＋/CD8 ^＋ from all monkeys became inverted from 28d after inoculation. Conclusion It has succeeded to inoculate rhesus monkeys with SIVmac251 strain by different routes, and for the first time in China, to be successful in establishment of a SAIDS animal model infected by a mucosal route.