摘要
[目的]建立一种测定苏丹红I的新方法。[方法]人血清白蛋白(HSA)与苏丹红I结合,用共振瑞利散射光法进行苏丹红I的检测。[结果]在360nm处共振光散射增强强度与苏丹红I的浓度呈线性关系。以K2HPO4-NaH2PO4为缓冲体系维持苏丹红I测定溶液的pH=5.80,当温度为305.16K(28℃)和静置时间为30min时,方法的检出限为0.10μg/ml,线性范围为0.10～2.80μg/ml,线性相关系数r=0.995,方法精密度(RSD)和准确度(P),分别为2.98%～5.19%和92.50%～108.00%。[结论]方法可靠、简便,快速,可直接测定水样中的苏丹红I。
[Objctive] To set up a new method for determination of sudan Ⅰ in hot chilli and related products. [ Methods] The sudan Ⅰwas determined by resonance light scattering （RLS）method after it had combined with human serum albumin（HSA）.[ Results] The enhanced intensity of RLS at the wavelength of 360 nm was proportional to the concentration of sudan Ⅰ.The buffer solution K2HPO4-- NaH2PO4 was used to maintain the solution pH5.80, When the temperature and place time were 305.16K （28℃）and 30 minutes, respectively, the linear range of the calibration curve was 0.10-2.80 μg/ml with the detection limit of 0.10 μg/ml and the correlation coefficient was 0.995.The RSD and the rates of recoveries were 2.98%- 5.19%and92.50%-108.00% respectively. [Conclusions] This method is reliable and accurate and can be used to detect sudan μ directly in hot chilli and related products.
出处
《现代预防医学》
CAS
北大核心
2007年第3期528-529,共2页
Modern Preventive Medicine
基金
广州市海珠区科技局立项项目(2006-D-11)
