摘要
本文利用A-PAGE方法对87份供试的小麦材料进行了黑麦碱(secalin)检测,并进一步利用低分子量麦谷蛋白Glu-B3的STS-PCR标记以及与抗条锈病基因Yr9紧密连锁的Xgwm582标记对上述材料进行了1BL/1RS易位检测。结果表明,在87份材料中鉴定出了一种新的改良1BL/1RS易位系,该类易位系的特征是:含有来自1RS的抗条锈病基因Yr9、不含黑麦碱、具有Glu-B3基因、比一般1BL/1RS易位系具有明显偏高的SDS-沉降值。本研究中选用的生化标记和分子标记可以有效检测出育种材料中的1BL/1RS、非1BL/1RS以及改良1BL/1RS易位系。
The presence of secalin was determined by A-PAGE and 1BL/1RS tnmslocation was characterized by PCR markers in 87 wheat materials in this study, The markers applied were Glu-B3 STS-PCR marker and Xgwm582marker which tightly linked with stripe rust resistance gene Yrg. The results indicated that an improved 1BL/1RS translocation has Glu-B3 and Yr9 without secalin, and has higher SDS- sedmentation value than common 1 BL/1RS translocation. The result also showed that the biochemical and molecular markers in this research could be applied to identify the presence of 1BL/1RS translocation as well as the type of such translocation.
出处
《西南农业学报》
CSCD
2007年第1期15-18,共4页
Southwest China Journal of Agricultural Sciences
基金
中国科学院知识创新工程重要方向
四川省科技厅项目资助
