丽江山慈姑秋水仙碱HPLC测定

Determination of colchiane in Iphigenia indica Kunth et Benth. by HPLC

采用HPLC法测定丽江山慈姑鳞茎不同部位及云南省西北部和四川省越西县野生居群秋水仙碱含量。结果表明:丽江山慈姑地上部分基本不含秋水仙碱,鳞茎秋水仙碱含量由外及内依次降低,其中外膜质层为0.134%和鳞茎外层为0.171%;22个野生丽江山慈姑居群中,丽江红岩(GS01)含量最高,达0.61%,永胜板桥乡(GS22)最低,为0.13%,平均秋水仙碱含量为0.40%。

Simulataneous HPLC determine the cdchicine in different part of bulb of Iphlgenia indica Kunth et Benth. and the wild population of the northwestward of Yunnan province and Yuexi county in Sichuan. The result shows：The up-ground parts of Iphigenia indica Kunth et Benth. contain few colchicine. The colchicine gradually reduce from outside to inner in the bulb of Iphigenia indica Kunth et Benth. , the outer-epidermis contain 0.134 % ,the outer of bulb contain 0. 171%. The content of colchicine in the twenty-two wild populations GS01 is 0. 61% and GS22 is 0.13 %. The average conents of these colchicine is 0.40 %.