摘要
目的:观察叶酸(FA)、VB6、VB12对同型半胱氨酸(Hcy)致血管内皮细胞损伤的交互保护作用。方法:原代培养大鼠主动脉内皮细胞(RAEC),根据正交分析结果分为6组,阴性对照组、阳性对照组(Hcy)、维生素对照组(FA+VB6+VB12)及三组维生素防护组(Hcy+FA,Hcy+FA+VB12,Hcy+FA+VB6+VB12),维生素用含终浓度为1.0mmol/L Hcy的培养基配制。干预24 h后,取上清液,通过MTT细胞增殖实验比较各组SOD、GSH-PX、MDA、NO、NOS等指标的差异。结果:叶酸与VB12能缓解1.0mmol/L Hcy对RAEC增殖的抑制作用,而VB6则无明显效果。叶酸与VB6无交互作用,与VB12存在交互作用;VB6与VB12之间也存在交互作用;FA、FA+VB12、FA+VB6+VB12三种组合均能拮抗Hcy对SOD、GSH-PX、NOS酶活性的抑制作用,保护内皮细胞功能。结论:同时补充叶酸、VB6、VB12可能是三者在体外保护Hcy损伤内皮的较理想组合。
Objective： To investigate the cross protective effects of folic acid, VB6, VB12 on rat aortic endothelial cells （RAEC） from injury induced by DL-homocysteine （Hcy）. Method： RAEC were cultured in vitro, and the cross effects of folic acid, VB6,VB12 were studied by MTT. According to the results of orthogonal design, the activities of SOD, GSH-Px, NOS and the contents of MDA, NO in the media were compared. Results： The results of orthogonal design indicated that the inhibition of Hcy to RAEC could be relieved by folic acid, VB12 excluding VB6. The interaction existed between folic acid and VB12, as well as VB6 and VB12. The inhibition of Hcy to the enzymes （SOD, GSH-PX, NOS） could be relieved by three vitamin projects. Folic acid, VB12, VB6 could inhibit the reduction of NO and decrease the production of MDA. Conclusion-. The simultaneous addition of folic acid, VB6 and VB12 may be an excellent strategy for protection of RAEC injury induced by DL-homocysteine.
出处
《营养学报》
CAS
CSCD
北大核心
2007年第1期9-12,共4页
Acta Nutrimenta Sinica
基金
中国营养学会营养科研基金2004年资助项目
