辛基酚和三羟异黄酮对大鼠乳腺癌ER、AIB1和PCNA表达的影响

EFFECTS OF OCTYLPHENOL AND GENISTEIN ON ER,AIB1,AND PCNA EXPRESSIONS IN RAT BREAST CANCER

目的:探讨辛基酚和三羟异黄酮对大鼠乳腺癌激活因子(AIB1)、雌激素受体(ER)和增殖细胞核抗原(PCNA)表达的影响。方法:动物随机分为正常对照组(Con)、二甲苯恩(DMBA)诱导乳腺癌模型组(Mod)、三羟异黄酮组(GEN)、辛基酚组(OP)和联合作用组(GP),采用RT-PCR和免疫组化检测正常乳腺组织和乳腺癌组织中AIB1、ER和PCNA的mRNA或蛋白表达水平。结果:与Con组比较,Mod组乳腺癌组织中AIB1和ER mRNA表达水平上调,PCNA、ER表达量增加。与Mod组比较,GEN组乳腺癌组织中AIB1、ER mRNA及ER表达量明显降低,而OP组AIB1、ER mRNA和ER、PCNA表达水平则明显增加。与OP组比较,GP组能部分降低乳腺癌组织中AIB1、ER mRNA和ER、PCNA的表达量。结论:OP上调乳腺癌组织中AIB1、ER、PCNA的表达使二甲苯蒽诱导乳腺癌的发生率增加,而GEN及其与OP联合作用可下调AIB1、ER和PCNA的表达量,降低诱导乳腺癌的发生。

Objective： To study the effects of octylphenol（ OP）and genistein（ GEN ）on the expressions of estrogen receptors （ER）, amplified in breast cancer 1（AIB 1） and proliferating cell nuclear antigen （PCNA） in rat breast cancer. Method： Female SD rats were randomly divided into control, model, GEN treated, OP treated, GEN and OP combined treated groups. RT-PCR and immunohistochemical methods were used to detect the expressions of AIB1, ER, PCNA on normal mammary gland and mammary cancer. Results： Compared with control mammary gland, AIB1 mRNA, ER mRNA, PCNA and ER expressions were up-regulated in mammary cancer. Compared with mammary cancer in model group, the level of AIB ImRNA, ER mRNA and ER expressions were significantly decreased in GEN treated group, while they were significantly increased in OP treated group and PCNA expression was significantly increased too. Compared with OP treated group, the level of AIBlmRNA, ER mRNA, ER and PCNA expressions were partly decreased in GEN and OP combined treated group. Conclusion： Octylphenol can up-regulate the levels of AIB 1, ER and PCNA expressions in mammary cancer and may increase the incidence of 7,12- dinmethylbenz[a]anthracene （DMBA）-induced mammary cancer. Genistin or genistein combined with octylphenol can downregulate the levels of AIB1, ER and PCNA expressions in mammary cancer, and inhibit their incidences.