氟对体外培养的血管内皮细胞增殖的影响

Effect of Fluoride on Proliferation of Human Blood Vessel Endothelial Cell in Cultured

目的观察不同浓度的氟对体外培养的人脐静脉血管内皮细胞增殖的影响。方法利用二甲基噻唑二苯基四唑溴(MTT)比色法、流式细胞仪及免疫组织化学法观察不同浓度的(120、240、360、480、600、720、840、960μmol/L)氟化钠染毒24h对体外培养的人脐静脉血管内皮细胞增殖活性的影响。结果氟化钠的浓度在120～240μmol/L时,增殖细胞核抗原表达阳性率高于正常对照组(P<0.01),240μmol/L时的阳性率最高约为80%;吸光度(A)值和细胞分裂增殖指数(PI)值也高于正常对照组(P<0.05,P<0.01);360～480μmol/L氟化钠染毒组上述指标与正常对照组差异无统计学意义(P>0.05);当氟化钠的浓度增加到600μmol/L以上时,增殖细胞核抗原的表达、A值及PI值均低于正常对照组(P<0.05,P<0.01),以960μmol/L时增殖抗原表达阳性率最低(约为20%),并且随着浓度增高上述指标呈下降趋势。结论低浓度的氟化钠能促进脐静脉血管内皮细胞增殖活性,高浓度时抑制脐静脉血管内皮细胞的增殖活性,且随着氟浓度的增加,抑制作用逐渐增强。

Objective To observe the effects of fluoride of different dose on the proliferation of the cultured human umbilical vein endothelial cells（HUVEC）. Methods The cells were treated with NaF at different doses. Cell counting, MTT colorimetry, flow cytometry （FCM） and immunohistochemistry were used to detect the endothelial cells activities. Results Compared with the fluoride free control, the positive rate of PCNA and Ki-67 were higher as NaF concentration was at 120-240 μmol/L and were highest at 240 μmol/L, the same was seen in the values of A and PI, as NaF concentration was at 600-960μmol/L,these indexes decreased. Conclusion In vitro, the low concentration of fluoride can promote the proliferative activity of HUVEC, whereas the high concentration can inhibit it.