摘要
目的建立尿清颗粒中丹参酮ⅡA的含量测定方法。方法采用HPLC法,KromasilC18(250mm×4.6mm,5μm)色谱柱,流动相:甲醇-水(75∶25),检测波长:270nm,流速:1ml.min-1。结果丹参酮ⅡA线性范围为0.528～2.64μg,回收率(n=6)为99.14%,RSD=1.37%。结论本方法分离效果好,准确可靠,是控制尿清颗粒内在质量的有效方法。
OBJECTIVE To establish the determination of tanshinone ⅡA in Niaoqing Granules. METHODS HPLC method was developed for the determination of tanshinone ⅡA in Niaoqing Granules on Kromasil C18 （250min×4.6mm, 5μm）, using methane-water （75 ： 25, v ： v） as mobile phase （1.0ml·min^-1） and detection wavelength was 270nm. RESULTS The linear range of tanshinone ⅡA was 0.528～2.64μg and the correlation coefficient was 0.9999, the average recovery was 99.14%, RSD was 1.37% （n = 6）. CONCLUSION The method was convenient and accurate. It can be used for the quality control of Niaoqing Granules.
出处
《齐鲁药事》
2007年第2期86-88,共3页
qilu pharmaceutical affairs
