摘要
本文研究了酯酶在小菜蛾Ｐｌｕｔｅｌａｘｙｌｏｓｔｅｌａ抗药性中的作用。离体酯酶活力测定表明，抗性品系（ＧＺ）幼虫的羧酸酯酶活力是敏感品系（ＳＳ）的１．４倍，故羧酸酯酶在抗性品系（ＧＺ）抗药性的作用不大；酸性和碱性磷酸酯酶在抗性和敏感品系的幼虫中差异很小，幼虫的酸性和碱性磷酸酯酶电泳酶谱没有差异。幼虫酯酶同工酶电泳分析显示Ｅｓｔ－１位点的Ｃ基因的频率随着抗性水平的提高而增加，基因型的多态性也提高。
Polyacrylamide gel electrophoresis and enzymatic determination in vitro were used to demonstrate the relationship be esterase activity and resistance in diamondback moth. The results are briefly stated as follows:The carboxylesterase activity were stronger in the resistant strain than in the susceptible strain. No difference was observed in acid and alkaline phosphatase, not only in enzgmatic determination in vitro but also in the zymograms. Esterase zymograms from polyacrylamide gel electrophoresis revealed higher frequency of C allele and more genotype in Est 1 loci in resistance strain.
出处
《昆虫天敌》
CSCD
1996年第4期23-29,共7页
Natural Enemies of Insects
基金
广东省科学基金
广东省招标课题资助
