母兔超数排卵方法的研究

Study on Superovulation in Female Rabbits

采用相同品种、月龄和体重的处女母兔进行超数排卵试验。ｈＣＧ剂量为５０ＩＵ／ｋｇ时，按每公斤体重肌注不同的ＰＭＳＧ剂量：２０ＩＵ、３０ＩＵ、４０ＩＵ、５０ＩＵ和６０ＩＵ，平均每只母兔胚胎数（Ｎ分别为：１０．０±５．７、１６．３±１２．０、２０．１±７．３、１７．５±９．９和９．７±４．７，ＰＭＳＧ为４０ＩＵ／ｋｇ的超排效果最好。当ＰＭＳＧ４０ＩＵ／ｋｇ时，ｈＣＧ剂量分别为５０ＩＵ／ｋｇ、７０ＩＵ／ｋｇ和９０ＩＵ／ｋｇ，Ｎ相应地为２２．３±１０．５、２２．８±１４．８和２６．０±１８．４随着ｈＣＧ剂量升高，超排效果逐渐改善，但三者之间差异不显著。结果表明，过低或过高剂量的ＰＭＳＧ，显著降低母兔的超排效果，ｈＣＧ剂量的提高，有助于提高排卵率，获得较多的胚胎。

Superovulation induced with hCG and PMSG was performed in vir-ginal rabbits whose breed,age and body weight were limited under same conditions.Before intravenousl injection with hCG unchangeably at 50IU / kg,rabbits were treat-ed by intramuscular injection with dif ferent dosages of PMSG at 20IU,30IU,40IU,50IU and 60IU per kilogram body weight.The corresponding average value in numberof embryos frOm every group superovulation-treated donors was 10.0±5.7,16.3±12.0,20.1±7.3,17.5± 9.9 and 9.7± 4. 7 respectively.As a result,the effectivesuperovulation was significantly elicited with PMSG at 40IU / kg as hCG at 50IU/ kgstrictly.Similarly, with PMSG being given at fixed dosage,40IU / kg,superovulatorytreatment was carried out with different dosage of hCG at 50IU / kg,70IU / kg and90IU/ kg separatively,the average value ofobtained embryos(AVE)were 22.3±10.5,22.8± 14. 8 and 26.0± 18.4 respectively.With the increase of dosage of hCG, thesuperovulatory effect gradually showed some improvement,but no significant differ-ences among three AVEs were indicated.The conclusion demonstrated that lower orhigher dosage of PMSG negativly affected the superovulatory performance in rabbits;whereas increasing at dosage of hCG had the advantage of that at dosage of PMSG inovulating rate and harvesting embryos.