塞来昔布对人食管癌细胞株Eca-109增殖和凋亡的影响

Effects of Celecoxib on the Proliferation and Apoptosis of Human Esophageal Carcinoma Cell Line Eca-109

目的体外研究选择性环氧化酶-2(COX-2)抑制剂塞来昔布(celecoxib)对人食管癌细胞株Eca-109增殖和凋亡的影响,并探讨其可能的作用机制。方法采用噻唑蓝(MTT)比色法、流式细胞仪(FCM)、吖啶橙染色结合荧光显微镜观察细胞形态等技术,研究塞来昔布对Eca-109细胞增殖的抑制和促进凋亡的影响。结果MTT比色法显示塞来昔布对人食管癌Eca-109细胞有较强的抑制作用,呈时间和浓度依赖性;FCM显示有凋亡峰出现,凋亡率在(15.89±0.87)%^(73.57±1.63)%;塞来昔布使G0/G1期细胞比例升高,S期和G2/M期比例下降,呈一定效应关系;荧光显微镜下可见典型的细胞凋亡形态学特征:细胞体积缩小、细胞核固缩,凋亡小体形成等。凋亡比例呈剂量和时间依赖。结论塞来昔布体外能够显著抑制Eca-109增殖,诱导凋亡,可能与诱导凋亡和阻止细胞周期进展有关。

Objeetive To determine the effect of Celecoxib on the proliferation and human esophageal carcinoma cell Line Eca - 109 and the probable mechanism involved. Methods Using Methabenz- thiazuron （MTY） assay, flow cytometry （FCM） , Acridine orange staining. The effects of celecoxib on the proliferation and apoptosis of Eca - 109 cells as well as its related mechanism were studied. Results The growth of Eca - 109 cells was inhibited apparently by celecoxib in a time and dose dependent manners. Sub - G1 peak was detected by FCM, and apoptotic rate of which was （ 15.89 ±0.87）% （73.57 ±1.63）%. The cell ratio of GO/ G1 phase increased, whereas the cell ratio of S and G2/M phases decreased after treatment, which was in a dose - dependent manner as well. The treated Eca - 109 cells exhibited some morphological features of apoptosis, including cell shrinkage, nuclear condensation, DNA fragmentation, and the formation of apoptosis bodies, the apoptotic index of which was in a time and dose dependent manners. Conclusions tosis of Human Esophageal Carcinoma Cell Line Eca cle progress of Eca - 109 ceils. Celecoxib inhibited proliferation and induced apop- - 109, which may be related to blocking the cell cy cle progress of Eca - 109 ceils.