壳聚精固定α－淀粉酶的研究（I）

STUDIES ON LMMOBITIZATION OF α- AMYLASE ON DEACETYLATED CHITIN(I)

用壳聚糖作载体，戊二醛为交联剂，采用吸附交联法将α－淀粉酶固定化，研究了固定化条件和固定化酶的性能．在酶浓度８ｇ／Ｌ，６～８℃，ｐＨ６．８，吸附１２ｈ，ｌ％戊二醛交联４ｈ的固定化条件下，固定化效果较优．固定化酶的酸碱稳定性、热稳定性、贮存稳定性较好．最适作用ｐＨ较自由酶酸移，最适作用温度及Ｋｍ值较自由酶高，同时显示出一定的操作稳定性．

?Amylase was immobilized on deacetylated chitin and crosslinked with glu taraldehydes.Enzyme concentration:8 mg/ml,temperature: 6～8°C,pH 6.8,absorbing time: 12 h,crosslinking time:4 h,glutaraldehydes concentration: 1 % was the optium condition for immobilization.The stability to pH,temperature,storage and the optium pH,the optium tem perature,the operational stability of immobilized α- amylase were studied.