摘要
将稳定转染了大鼠酪氨酶羟化酶（Ｔｙｒｏｓｉｎｅｈｙｄｒｏｘｙｌａｓｅ，ＴＨ）基因的大鼠成肌细胞移植于帕金森病大鼠模型的纹状体，进行基因治疗研究。ＲＴ－ＰＣＲ和免疫组织化学检测都证明转基因细胞可在纹状体内存活并表达ＴＨ，动物的不对称旋转行为明显改善，而且疗效可维持半年以上。
Transgenic myoblast cell line L-6TG-TH which stably expresses rat tyrosine hydroxylase(TH) gene was transplanted into the striatum of the Parkinson disease (PD) rat model. RTPCR and immunohistochemical staining show that such transgenic myoblasts could survive and express TH gene in vivo. The asymmetrical rotation of PD rats model was reduced significantly by 65% and the effect lasted for at least 6 months.Other authors: Zhao Yin-Chun and Chen Sheng-Di (Rui Jin Hospital, Shanghai 200025 );Jiang Zhi-Hua and Zhou Chang-Fu (Shanghai Institute of Physiology, Academia Sinica, Shanghai 200031)
基金
863计划项目
国家自然科学基金
关键词
基因治疗
动物实验
震颤性麻痹
Parkinson disease, Gene therapy, Tyrosine hydroxylase, Myoblast cell line, Intracerebral transplantation
