酰化人红细胞超氧化物歧化酶稳定性的研究

Study on Stabilities of Acylated Human Erythrocyte Superoxide Dismutase

用月桂酸对人红细胞超氧化物歧化酶（ｈ－ＳＯＤ）进行化学修饰得到酰化ｈ－ＳＯＤ（Ａｃ－ｈＳＯＤ），并对Ａｃ－ｈＳＯＤ和ｈ－ＳＯＤ的稳定性进行了比较。结果表明：Ａｃ－ｈＳＯＤ活力为ｈ－ＳＯＤ的７２％。比活力为４０００Ｕ／ｍｇ，Ａｃ－ｈＳＯＤ的热稳定性、酸碱稳定性及抗蛋白酶水解能力均比天然酶提高。

The acylation of human erythrocyte superoxide dismutase with lauric acid was performed,and some of the stabilities of acylated and native enzyme were also studied. The results indicated that the acylated enzyme retained 72% of the original enzyme activity and its specific activity was 4000 U/mg. The acylated enzyme showed greater resistance to trypsin degradation, enhanced heat and PH changes.