葡聚糖磁性毫微粒固定化L－天冬酰胺酶的研究

Study on Immobilization of L-Asparaginase on Dextran Magnetic Nanoparticles

葡聚糖磁性毫微粒固定化Ｌ－天冬酰胺酶的研究徐慧显，李民勤，潘再群，马建标，何炳林（南开大学高分子化学研究所，天津３０００７１）大肠杆菌天冬酰胺酶对急性淋巴白血病有明显疗效［１］，注射入体内以后，可迅速清除血清中的天冬酰胺──敏感性肿瘤细胞的必需营养成...

Superparamagnetic dextran magnetic nanoparticles with a mean diameter of 313 nm were prepared by coprecipitation. Effects of concentration of NaIO_4, amount of enzyme and reaction time in the process of preparation on the activity of enzyme immobilized on dextran magnetic nanoparticles were studied. Effects of temperature and chymotrypsin on immobilized L-asparaginase were compared with those of soluble enzyme. After L-asparaginase was immobilized,its thermal stability was improved slightly, and its resistance to hydrolysis by chymotrypsin was increased remarkably.