摘要
葡聚糖磁性毫微粒固定化L-天冬酰胺酶的研究徐慧显,李民勤,潘再群,马建标,何炳林(南开大学高分子化学研究所,天津300071)大肠杆菌天冬酰胺酶对急性淋巴白血病有明显疗效[1],注射入体内以后,可迅速清除血清中的天冬酰胺──敏感性肿瘤细胞的必需营养成...
Superparamagnetic dextran magnetic nanoparticles with a mean diameter of 313 nm were prepared by coprecipitation. Effects of concentration of NaIO_4, amount of enzyme and reaction time in the process of preparation on the activity of enzyme immobilized on dextran magnetic nanoparticles were studied. Effects of temperature and chymotrypsin on immobilized L-asparaginase were compared with those of soluble enzyme. After L-asparaginase was immobilized,its thermal stability was improved slightly, and its resistance to hydrolysis by chymotrypsin was increased remarkably.
出处
《生物化学杂志》
CAS
CSCD
1996年第6期744-746,共3页
基金
国家自然科学基金"八五"重大项目
关键词
L-天冬酰胺酶
葡聚糖
磁性毫微粒
Dextran magnetic nanoparticles, L-Asparaginase, Immobilized enzyme