建立三种基因工程杀蚊幼蓝藻

ESTABLISHMENT OF THREE KINDS OF GENETICALLY ENGINEERIED LARVICIDAL CYANOBACTERIA

基因工程杀蚊幼蓝藻的应用，为持续控制蚊幼虫的研究提供了新的途径。本文将携有球形芽孢杆菌晶体蛋白基因的ｐＤＣ２６穿梭质粒，通过接合转移导入多种鱼腥藻中进行表达。结果表明，所得到的基因工程藻Ａｎａｂａｅｎａｓｐ．７１２０Ａｎａｂａｅｎａｃｙｌｉｄｒｉｃａ和Ａｎａｂａｅｎａｓｕｂｔｒｏｐｉｃ对淡色库蚊幼虫具有较高的毒效，２４ｈ半数致死浓度（ＬＣ５０）分别为：３．５１×１０４细胞／ｍｌ、３．３２×１０４细胞／ｍｌ和４．３４×１０４细胞／ｍｌ，其杂合质粒在蓝藻中有很高的稳定性，具备现场应用的要求。

Three kinds of high larvicidal activity of recombinant Anabaenas, Anabaena sp. 7120, A. cylidrica and A. subtropic, were developed by expressing the toxin genes of Bacillus sphaericus, their median lethal concentration (LC 50 )(24h) to larvae of Culex pipiens pallens were 3.51×10 4 cells/ml, 3.32×10 4 cells/ml and 4.34×10 4 cells/ml respectively, and the plasmid pDC26 was high stability. This research provided new ways for control mosquito.