摘要
将10%福尔马林1.0~1.5ml经尿道注入大鼠膀胱,于刺激后的0.5,1,2,8,24,48h取材,进行兔疫组化(ABC法)反应,观察脊髓腰骶段后连合核区的SP样纤维和终末的分布,并随机抽样进行图像分析、另设对照组,向膀胱内注入生理盐水,0.5,2.0h后取材。结果显示:福尔马林刺激后,腰骶髓后连合核区SP样阳性纤维和终末与对照组相比,在lh内明显减少,而在2h时则呈增加趋势,8h又恢复正常。同时本文以同样动物模型,实验组分别于刺激后1,3,8,12,24,48h和5d取材,对照组于刺激后24h取材,进行原位杂交组化反应,观察腰骶髓背根神经节内SPmRNA阳性神经元的数量。结果显示:福尔马林刺激后3h即出现SPmRNA表达神经元数量的明显增加,24h达到高峰,并在48h以后开始下降,到5d后回落到正常水平。
Changes of substance P-like immunoreactivity (SP-LI) in the lumbosacral dorsal commissural nucleus (DCN) were in-spected microscopically and semiquantified with a computer-assisted image processing system in rats with different times (0.5 h, 1 h, 2 h, 8 h, 24 h, 48h) after injection of 1. 0~1.5 ml 10% formalin into the bladder via the urethra. The resultsshowed that SP-LI in DCN significantly decreased from 0. 5 hour to 1 hour after injection, wereas SP-LI significantly in-creased at about 2 hours after the injection. After 8 hours, the levels of SP-LI was almost equal to that of the controlgroups. We also inspected the changes of SP-mRNA expression in the lumbosacral dorsal root ganglian(DRG) neurons andsemiquantified microscopically with different times (1 h, 3 h, 8 h , 24 h, 48 h, 5 d) after the same injection in situ. The re-sults showed that the numbers of SP mRNA-POsitive neurons in DRG significantly increased from 3 hours after injection.The SP mRNA-positive neurons increased to the peak at 24 hours (P<0.01). After 5 days of injection, the numbers of SPmRNA positive neurons decreased to the level of contral groups.
出处
《神经解剖学杂志》
CSCD
北大核心
1996年第3期239-244,共6页
Chinese Journal of Neuroanatomy
关键词
腰骶髓后连合核
背根神经节
P物质
神经解剖
Substance P
SP mRNA
dorsal commissural nucleus
dorsal root ganglion
immunohistochemistry
in situ hy-bridization
rat