移植的齿状回颗粒细胞在培养的大鼠海马组织上移行特征

MIGRATION OF GRAFTED DENTATE GRANULE CELLS IN RAT HIPPOCAMPAL SLICE CULTURE

为探讨齿状回颗粒细胞在培养的海马组织表面上的移行过程以及苔状纤维的投射特征,本实验从生后3d的绿色荧光蛋白(GFP)基因导入的SD大鼠海马组织切片中分离出齿状回颗粒细胞层,移植到培养的SD大鼠海马组织切片表面上,共同培养3～4d后,激光共聚焦显微镜观察。结果显示,97.6%的细胞移行到齿状回和CA3区,仅有2.4%细胞移行到CA2和CA1区。移行到齿状回颗粒细胞层的颗粒细胞投射苔状纤维到齿状回门区和CA3区的透明层,却不到CA2和CA1区。上述结果提示,在海马组织培养中移植的齿状回颗粒细胞的移行以及苔状纤维的投射方向明显受宿主的影响。

The present study was designed to investigate the characteristics of migration and mossy fiber projection of grafted dentate granule cells in hippocampal slice culture. Stratum granulosum of hippocampal dentate gyrus from 3-day-old transgenie SD rats expressing green fluorescent protein （GFP） was grafted onto the SD rat hippocampal slices and cultured for 3 -4 days. Laser eonfocal microscope was used to trace migration and mossy fiber projection of the dentate granule cells. The results showed that about 97.6% of transplanted dentate granule cells migrated into the area of dentate gyrus and CA3 sector while only 2.4% cells migrated into the CA2 and CA1 sectors. The migrated granule cells projected mossy fibers into the dentate hilus and lueidum of CA3 sector but did not project to the CA2 and CA1 sectors. The present results suggest that migration and mossy fiber projection of the grafted dentate granule cells are depend on the host environment of the rat hippocampal slice cultures.