GAD67-GFP转基因小鼠三叉神经脊束核吻侧亚核和极间亚核内GABA和甘氨酸共存神经元的观察

COEXISTENCE OF GABA AND GLYCINE IN THE NEURONS OF THE ORAL AND INTERPOLAR SUBNUCLEI OF THE SPINAL TRIGEMINAL NUCLEUS IN THE GAD67-GFP TRANSGENIC MOUSE

本文以GAD67-GFP基因敲入小鼠为研究工具,利用免疫荧光组织化学双重染色技术观察了小鼠三叉神经脊束核(spi-nal trigeminalnucleus,V)的吻侧亚核(Vo)和极间亚核(Vi)神经元内谷氨酸脱羧酶67[GAD67,γ-氨基丁酸(GABA)能神经元的标识物质]和甘氨酸(glycine,Gly)的定位分布和共存情况。结果显示,在Vo和Vi内均有比较密集分布的GAD67和Gly阳性神经元。此外,在Vo和Vi内也可见一些GAD67阳性神经元同时呈Gly阳性反应,即存在GAD67/Gly双标神经元。结果表明,Vo和Vi内有GABA和Gly共存神经元,它们可能在初级传入信息的传递和调制中发挥重要作用。

The present study was designed to observe the distribution and coexistence of glutamic acid decarboxylase 67 [GAD67, the marker of the γ-aminobutyric acid （GABA） -ergic neuron] and glycine （Gly） in neurons of the oral subnucleus （Vo） and interpolaris subnucleus （Vi） of the spinal trigeminal nucleus （V） in the GAD67-green fluorescent protein （GFP） transgenic mouse. GAD67-GFP knock- histochemical double-stalning technique were used. The results showed that GAD67 （GAD67-IR） and Gly-IR neurons were quite densely distributed in the Vo and Vi. Some of the GAD67-IR neurons also showed Gly-immunoreactivities, i.e. there were GAD67/GIy double-labeled neurons, in the Vo and Vi. The present results indicate that there are GABA and glycine co-existed neurons in the Vo and Vi. They might exert important effects on the transmission and regulation of the primary sensory information.