人体肿瘤细胞系在严重联合免疫缺陷（SCID）小鼠的爪垫和皮下移植后侵袭和转移的研究

Study on Invasion and Metastasis of Human Tumor Cells After Subcutaneous Transplantation of Tumor Cells into Different Sites of SCID Mice

本文用人体肺腺癌（ＬＴＥｐ－ａ·２）和人体鼻咽癌（ＣＮＥ－２Ｚ）两株细胞系，在严重联合免疫缺陷（ＳＣＩＤ）小鼠的背侧皮下和后肢爪垫移植：（１）爪垫移植组，两种肿瘤细胞系各用６只ｓｃｉｄ小鼠，分别于每只动物左后肢爪垫移植２个瘤组织小块；同一只动物右后肢爪垫接种２．５×１０￣６／０．０３ｍｌ瘤细胞悬液。（２）背部皮下移植组：两种肿瘤亦各６只：ｃｉｄ小鼠，在每只动物的左侧背部皮下移植２个瘤组织小块；对侧皮下接种２．５×１０￣６／０．０３ｍｌ瘤细胞悬液。２４只ｓｃｉｄ小鼠分４８个部位同时移植，动物均于肿瘤移植后６８天全部处死。实验结果：两种肿瘤在ｓｃｉｄ小鼠的背部皮下无论是用瘤组织小块或瘤细胞悬液移植，镜下均能见到肿瘤生长，而在爪垫移植后除了ＣＮＥ－２７瘤悬液接种的右侧３只爪垫未见肿瘤结节外，其他亦均见肿瘤生长。组织学观察，ＣＮＴ－２７细胞无论是用瘤小块或瘤悬液在背部皮下移植，局部一般都发生不同程度的侵袭，有３／６例（５０％）发生肺内转移；爪垫移植仅瘤小块移植组全部呈侵袭性生长，瘤悬液接种均未见侵袭发生；瘤小块移植组有４／６例（６６％）发生同侧腹股沟淋巴结转移。ＬＴＥＰ－ａ．２细胞系无论是在哪一部位或用哪种方式移植?

LTEP-a-2 human lung adenocarcinoma cells and CVE-2Z human nasopharygcal carcinoma cells were usedin prcsent experiment.Tumor cells were injected into different sites(including site of subeutaneous costal region and subcu-taneous footpad)and different ways(including inoculation of suspension of tumor cells and tumor fragment)into 24 scidrnice(severe combined immunodeficience mice)and 48 sitcs. Experiments were divided into two groups,named as group ofsuhcdtaneous inoclatuon into costal region(including 6 LTEP-a-2 tumor-bearing scid mice and 6 CNE-2Z tumor-bearing scidmice)and group of subcutancous inoculation into footpad(including 6 LTEP-a-2 tumor-bearing scid mice and 6 CVE-2Z tu-mor bearing scid mic).Two transplantable sites in cach mouse were chosen for this study,i.e the suspcnsion of tumorcclls was implanted into one site,and the tumor fragments were implanted into opposed site in same mouse:All tumor-bcar-ing scid mice were sacrificed on 68th day.In the group of subcutancous inoculation into costal rcgion,CNE-2Z tumor couldgive rise to growth(100%)with progrcssive invasion.and lung mctastasis which occurred in 50%(3/6).LTEP-a-2 tumorwere grown in the form of nodule with incomplete thinner fibrous capsule(100%).slight tumor invasions were noticed insome mice. metastasis was not observed In the group of subcutaneous inoculation into footpad,after inoculation of tumorfragment of CNE-2Z tumor cells into 6 scld mice tumor give rise to growth(100%)with progressive invasion and lymphaticnetastasis which occurred in 66%(4/6)of cascs,And after inoculation of suspcnsion of CNE-2Z tumor cells into 6 scidrnicetumor give rise to growth(50%)without invasion and mctastasis. In thc group of subcutancous inoculation LTEP-a-2 tumorcclls into footpad tumor growth was observed in the form of module with fibrous capsul(100%).and slight invatsion was pre-xented in the some cascs,metastasis was not observed In the solid tumors of some cases of CNE-2Z and LTEP-a-2 tumorsthere was an accompanying squarnous element.the mcchainsm was unclcar. These results showed that human tumor growthin seidmicc is obviously influcnced by the sites and was of inoculation