肝细胞癌及相关慢性肝病肝组织中乙型肝炎病毒的研究

Hepatitis B Virus in Liver Tissue of Hepatocellular Carcinoma and Related Chronic Liver Diseases

目的:初步探讨HBV感染与肝细胞癌之间的关系。方法:应用免疫组化和原位杂交分别在检测慢性肝炎、肝硬化、肝细胞癌及癌旁肝组织中HBsAg和HBVDNA的基础上,选择HBsAg和HBVDNA均为阳性的标本进行免疫组化与原位杂交双标记。结果:癌组织中HBsAg及HBVDNA阳性率低,信号少,且HBVDNA多在核内;而慢性肝炎、肝硬化和癌旁肝组织中HBsAg及HBVDNA阳性率高,信号多而强,HBVDNA可在胞浆或胞核。HBVDNA与HBsAg可共存于同一细胞或分布于不同肝小叶或同一肝小叶的不同部位。结论:大多数肝细胞癌的发生与HBV感染所致的慢性肝炎和肝硬化密切相关;双标记法有利于分析2种不同病毒标志物之间的关系。

Objective： To investigate the relationship between hepatitis B viral infection and hepatocellular carcinoma. Methods： Based on the detection of HBsAg and HBV DNA in the liver tissue of chronic hepatitis, liver cirrhosis, hepatoceUular carcinoma and paratumor by immunohistochemistry and in situ hybridization respectively, the cases with both HBsAg and HBV DNA positive were detected using double labeling technique. Results： The positive rates and signals of HBsAg and HBV DNA were greater in chronic hepatitis, liver cirrhosis and paratumor than those in hepatoceUular carcinoma. Most of HBV DNA in hepatoceUular carcinoma was in nucleus and HBV DNA in chronic hepatitis, liver cirrhosis and paratumor was in cytoplasm or nucleus. HBsAg and HBV DNA coexisted in a single cell or distributed in different liver lobules or different parts of the same lobule. Conclusion： Most of hepatoeellular carcinoma was associated with chronic hepatitis and liver cirrhosis infected by hepatitis B virus. Double labeling was a useful method to analyze the correlation of two different viral markers.