SELDI技术对马尔尼菲青霉菌酵母相和霉菌相蛋白质组表达差异的分析

Analysis of the difference in proteome expression between yeast form and mould form of Penicillium marneffei using SELDI technique

目的比较马尔尼菲青霉菌酵母相和霉菌相蛋白质组表达差异。方法应用表面增强激光解吸离子化飞行时间质谱技术(SELDI)检测马尔尼菲青霉菌酵母相和霉菌相的蛋白质谱。用PBSIIC型蛋白质芯片阅读机读取数据并进行分析。结果在WCX2芯片上共捕获75个蛋白峰,其中10个蛋白质在酵母相高表达,3个蛋白质在霉菌相高表达。相对分子质量为2900和3151蛋白质仅在酵母相表达,13151和13285蛋白质仅在霉菌相表达。结论SELDI技术可以检测出马尔尼菲青霉菌霉菌相和酵母相表达的小分子蛋白质差异。

Objective To compare the difference in protome expression between yeast form and mould form of Penicillium marneffei. Methods Surface enhanced laser desorption/ionlzation （SELDI） time-of-flight mass spectra were performed to compare the expressed proteins between yeast form and mould form of Penicilliurn marneffei. Protein profiling was read by PBSIIC ProteinChip Reader and the proteome database was analyzed by Proteinchip Software 3.2.0. Results Seventy-five distinct proteins were found in the yeast form and mould form of Penicillium marneffei, in which 10 proteins were up-regulated in yeast form and 3 in mould form. The proteins 2900 and 3151 were only expressed in the yeast form and the proteins 13 151 and 13 285 only in mould form. Conclusion SELDI technique can identify the difference of the expressed low-molecular-mass proteins between the mould form and yeast form ofPenicillium marneffei.