系统性红斑狼疮患者杀伤细胞免疫球蛋白样受体基因型分析

Genotype analysis of killer cell immunoglobulin-like receptors in systemic lupus erythematosus

目的探讨杀伤细胞免疫球蛋白样受体（KIR）基因型在系统性红斑狼疮（SEE）患者中的分布规律。方法采用序列特异性引物聚合酶链反应（PCR—SSP）法，分析93例SEE患者和123例无血缘关系的健康对照组KIR基因型。结果SEE组中3DL3＋，2DS2^-，2DL2^＋，2DL3^＋，KIRZ^＋，2DL1^＋，2DL4＋．3DL1^＋,3DS1^-，2DL5^-，2DS3^-，2DS5^-，2DS1^＋，2DS4^＋，3DL24^＋因型阳性率最高，占16．13％（与对照组比较P〈0．01），对照组中3DL3^＋，2DS2^-，2DL2^-，2DL3^＋，KIRZ^＋，2DL1^＋，2DL4^＋，3DL1^＋，3DS1^-，2DL5^-，2DS3^-，2DS5^-．2DS1^-，2DS4^＋，3DL2^＋因型阳性率最高，占19．51％（P=0．004），差异均有统计学意义。结论SLE与正常对照组中KIR基因型分布差异可能参与SLE发病。

Objective To investigate the association of killer cell immunoglobulin-like receptors （K1R） genotype with systemic lupus erythematosus （SLE）. Methods Using PCR-SSP to detect KIR genotype in 93 SLE patients and 123 randomly selected healthy controls. Results The genotype of 3DL3^＋, 2DS2^-, 2DL2^＋, 2DL3^＋, KIRZ^＋, 2DLI^＋, 2DL4^＋, 3DL1^＋, 3DS1^-, 2DL5^-, 2DS3^-, 2DS5^-, 2DS1^＋, 2DS4^＋, 3DL2^＋ were the most prevalent genotypes in the SLE group （16.13% in SLE vs 0.81% in controls, P〈0.01）; the genotypes of 3DL3^＋, 2DS2^-, 2DL2^-, 2DL3^＋, KIRZ^＋, 2DL1^＋, 2DL4^＋, 3DL1^＋, 3DS1^-, 2DL5^-, 2DS3^-, 2DS5^-, 2DS1^-, 2DS4^＋, 3DL2^＋ were the most prevelant in the controls （19.51% in controls vs 5.38% in SLE; P=0.004）. Conclusion The difference of KIR genotype between SLE patients and healthy controls may explain the pathogenesis of SLE.