不同表位60000SSA抗原免疫小鼠的反应

Reactions after mice immunized with multiple antigenic peptides of 60 000 SSA antigen

目的了解不同表位60000SSA抗原免疫小鼠后的反应，能否产生相应的抗体及不同的组织损伤。方法用60000SSA的多倍体寡肽抗原（MAPs）免疫小鼠，利用酶联免疫吸附法（ELISA）检测其是否产生了相应的自身抗体。并取其心肝肾等组织器官行病理学及免疫组织化学检查。结果各组小鼠均产生了抗多个表位的抗体．尤其是482～495组小鼠除产生抗482～495MAP抗体外，还对198-210组、212～219组、449～460组MAPs发生了免疫反应。但其余各组小鼠产生的抗体似无明显规律性。佐剂组小鼠及正常小鼠心、肝、肾脏免疫组织化学均呈阴性：482--495组、166～180组、401～411组小鼠心脏免疫组织化学均呈阴性，肝脏免疫组织化学80％呈阳性，部分小鼠肾脏免疫组织化学呈可疑阳性；各组小鼠心、肝、肾脏切片HE染色均未见明显形态学异常，与正常鼠无明显差异。结论MAPs具有免疫原性。经一种MAP免疫的小鼠可以产生对多种MAPs发生免疫反应的多克降抗体。并造成脏器损伤。

Objective To investigate the reactions after mice were immunized with multiple antigenic peptides （MAPs） of 60 000 SSA antigen. Methods Mice were immunized with different MAPs of 60 000 SSA and ELISA were used to detect anti-MAPs-antibodies in these mice. Lmmunohistochemical stain and HE stain were done to detect the anti-SSA autoantibodies and pathological damage in their hearts, livers and kidney. Results All groups of mice produced multiple-clone anti-MAPs antibodies, especially the mice of 482-495 group produced anti-MAP^9、10、20 antibodies as well as antibodies against other groups of MAPs. But the antibodies produced by the other groups of mice had no evident regularity. The immunohistochemical stain was negative in normal and adjuvant mice groups. In the 166-180, 401-411 and 482-495 groups of mice the immunohistochemical stain was negative in heart tissue was 80% positive in liver tissue and was suspiciously positive in kidney tissue. The HE stain was negative in all groups of mice. Conclusion MAPs have immunogenicity. Mice immunized with one group of MAPs can produced multi-clone antibodies and cause organ damage.