糖皮质激素受体α与激素量化治疗的初步研究

Study of the relationship between the expression of glucocorticoid receptor a and the dosage of glucocorticoid treating the patients with systemic lupus erythematosns

目的探讨31例系统性红斑狼疮（SLE）患者激素治疗前、后外周血单个核细胞（PBMCs）糖皮质激素受体α（GRet）的蛋白质水平、mRNA表达及其与疾病活动性的关系，分析GRot与SLE患者激素治疗疗效和剂量的关系。方法采用蛋白印迹（Western—blotting）和反转录-聚合酶链反应（RT-PCR）检测激素治疗前、后SLE患者和正常人外周血PBMCs中GRα蛋白质水平和GRαmRNA的表达，并将其与疾病活动性及激素剂量作相关分析。结果27例SLE患者激素敏感，4例激素耐药。激素敏感组PBMCs中GRα蛋白质水平和mRNA表达用药前高于用药后（P〈0．05），且与疾病活动性及激素剂量呈负相关。结论测定GRα的表达可能为激素的量化治疗提供依据。

Objective To investigate the expression of GRot in the peripheral blood mononuclear ceils （PBMCs） of 31 SLE patients before and after treated with glucocorticoid, in order to reveal the relationship between GRα and the dosage of glucocorticoid. Methods The expression of GRα mRNA and protein in PBMCs was detected by semi-quantitive reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting technique in SLE patients and healthy volunteer individuals. The relationship between GRα and SLEDAI score, the dosage of glucocorticoid was analyzed. Results Twenty-seven patients were sensitive and 4 patients were resistant to glucocorticoid. The expression of GRα mRNA and protein in the sensitive group with SLE who handn＇t been treated was higher than that of patients who had been treated with glucocorticoid （P〈0.05）, and associated negatively with SLEDAI score and the dosage of glucocorticoid. Conclusion The detection of GRα may provide a valuable scientific basis for the quantitive treatment witb glucocorticoid.