喉鳞状细胞癌中P53和PTEN蛋白的表达及临床意义

Expressions of P53 and PTEN in laryngeal squamous cell carcinoma and their clinical significances

目的 探讨喉鳞状细胞癌中P53、PTEN蛋白的表达及其与临床特征的关系。方法 采用免疫组化技术检测66例喉鳞癌组织和36例喉黏膜慢性炎症组织中P53、PTEN蛋白的表达。结果 P53、PTEN蛋白在66例喉鳞癌组织和36例喉黏膜慢性炎症组织中表达均存在统计学意义（P〈0．05）。66例喉鳞癌组织中，P53蛋白的表达水平在Ⅲ～Ⅳ期组高于Ⅰ～Ⅱ组（P〈0．05），淋巴结转移者高于无淋巴结转移者（P〈0．05）；PTEN蛋白的表达水平Ⅰ～Ⅱ期组高于Ⅲ～Ⅳ期组（P〈0．05），高分化癌组高于中低分化癌组（P〈0．05），无淋巴结转移者高于有转移者（P〈0．05）。喉鳞癌组织中P53、PTEN蛋白两者表达有相关性（P〈0．05）。结论 PTEN蛋白的表达对预测喉鳞癌的预后有一定参考价值。

Objective To investigate the relation of expressions of P53 and PTEN with clinical histological characteristics and prognosis of laryngeal squamous cell carcinoma （LSCC）. Methods The expressions of P53 and PTEN were examined with immunohistochemical staining in specimens from 66 cases of LSCC and 36 cases of laryngeal chronic inflammatory mucous membrane （LCIMM）. Results The differences of expressions of P53 and PTEN between LSCC and LCIMM were statistically significant （ P〈0. 05）. In LSCC the expressions of P53 protein in patients of stages Ⅲ-Ⅳ , with lymphatic metastasis were stronger than those of stages Ⅰ-Ⅱ ,without lymphatic metastasis respectively （ P〈0. 05） ; the expressions of PTEN protein in patients of stages Ⅰ-Ⅱ, well differentiation and without lymphatic metastasis were stronger than those of stages Ⅲ -Ⅳ, moderate to poor differentiation and with lymphatic metastasis respectively （ P 〈10.05）. There was no significant correlation between P53 protein and PTEN protein in LSCC （ P〉0. 05）. Conclusion The overexpression of P53 protein and lower expression of PTEN may play some important roles in the pathogenesis of LSCC.