摘要
目的 在体外用细胞生物学实验技术了解紫杉醇联合直线加速器X射线照射对Hep-2细胞株的作用，以及紫杉醇对Hep-2细胞株细胞周期的影响，并探讨其作用机制。方法 ①用MTT法计算Hep-2细胞经直线加速器照射组；先紫杉醇作用12、18、24、30h后再直线加速器照射组；先直线加速器照射再联合紫杉醇作用12、18、24、30h组各组细胞生长抑制率。②应用流式细胞仪检测Hep-2细胞经紫杉醇作用12、18、24、30h后各组细胞周期分布。结果 ①先紫杉醇作用再直线加速器照射组细胞生长抑制率较高（各组均值达到87．51％～89．64％），与先照射再联合紫杉醇组细胞生长抑制率（各组均值为57．84％～64．34％）及单纯照射组细胞生长抑制率（均值为41．90％）相比有统计学意义（P〈0．01）。②用紫杉醇后Hep-2细胞株细胞周期停滞于G2／M期，G2／M期停滞于24-30h达到峰值（24．65％～25．15％），并在用药后30h观察到凋亡峰。结论 紫杉醇有放射增敏作用，其可能机制是使细胞生长停滞于射线敏感期G2／M期，从而加强射线对肿瘤细胞的杀伤效应。
Objective To investigate the anti-tumor effect of taxol combined with accelerated radiation therapy and the effect of taxol on the cell cycle of Hep-2 cell lines, and to determine the mechanism of action of taxol combined with radiation in vitro. Methods ①The Hep-2 cell lines were divided into radiation group （radiation only）, taxol combined with radiation groups （exposed to taxol for 12, 18, 24, 30 h and then radiated） and radiation combined with taxol groups （radiated and then exposed to taxol for 12, 18, 24, 30 h）. The MTT assay was used to detect the growth inhibition rates of cell lines in all groups.②After Hep-2 cell lines were exposed to taxol for 12, 18,24,30 h, cell cycle analyses were performed by FCM. Results ①The cell growth inhibition rates of taxol combined with radiation groups （averaged from 87. 51% to 89. 64%） were much higher than those of radiation combined with taxol groups （averaged from 57. 84% to 64. 34%） and radiation group （41.90%）. The differences of cell growth inhibition rates were statistically significant （ P〈0. 01）. ②After exposed to taxol, the cells shifted from G0/C1 phases to G2/M phases depending on the exposure time. Apoptosis was induced after the cells were exposed to taxol for 30h. Conclusion Taxol is an active radiation sensitizer of Hep-2 cell lines. One of the mechanisms may be that it can accumulate the Hep-2 cell lines in G2/M phases in which cells are more sensitive to radiation.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2007年第1期17-20,共4页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
