姜黄素对大鼠脑缺血再灌注损伤的保护作用

Study on protective effects of curcumin on cerebral ischemia reperfusion

目的:研究姜黄素对大鼠脑缺血再灌注损伤的保护作用,并探讨其作用的机制。方法:采用线栓法制作大鼠大脑中动脉缺血再灌注模型,50只Wistar雄性大鼠被随机分为假手术组、缺血组和100mg/kg、300mg/kg姜黄素治疗组。缺血组和姜黄素治疗组分别在脑缺血再灌注后24h处死,观察大鼠神经功能缺失的评分,应用TTC染色观察梗死体积,应用TUNEL法及免疫组化染色检测脑组织凋亡细胞数及Bcl-2、Bax蛋白表达。结果:与损伤模型组比较,姜黄素治疗组改善大鼠神经功能的受损程度和减少脑梗死体积,各剂量姜黄素治疗组均可明显减少TUNEL染色阳性细胞数(P<0.01),明显增加Bcl-2蛋白表达(P<0.01),并且姜黄素治疗组Bax表达低于缺血组(P<0.05)。结论:姜黄素可通过上调Bcl-2蛋白下调Bax蛋白表达而减少脑缺血再灌注后神经细胞凋亡,从而改善受损的神经功能,对脑缺血再灌注损伤起保护作用。

Objective：to investigate the protective effects and mechanisms of curcumin after cerebral ischemia/ reperfusion（I/R） injury in rats. Method：The model of middle cerebral artery occlusion（MCAO） and reperfusion was set up using an intraluminal filament method. Fifty Wistar male rats were randomly divided into sham operation group,ischemic group and 100mg/kg,300mg/kg curcumin treated group.The rats in ischemic group and curcumin treated group were killed 24 hours after cerebral ischemia and reperfusion. We studied neurological scores and infarct size.Using the method of TUNEL and immunohistochemical staining to measure the number of neural apoptotic cells and Bcl-2,Bax protein expression. Result：Compared with the ischemic group, Curcumin decreased the neurological scores,infarct size and the number of neural apoptotic cells. The expression of Bcl-2 protein was increased significantly（P〈0.01） and the expression of Bax protein in curcumin treated group were decreased（P〈0.05）. Conclusion：Curcumin can decrease apoptosis by up-regulating Bcl-2 protein expression and down-regulating Bax protein expression. It has protective effect on the injury of ischemia and reperfusion.