荷兰菊组织培养快繁技术研究

Study on Tissue Culture and Rapid Propagation of Aster novi-belgii

本文利用组织培养技术研究荷兰菊的快速繁殖，为大量培育荷兰菊种苗提供可靠方法。通过多种培养基的试验筛选，找出较满意的培养方法、三种培养基依次使用，可以达到快速繁殖目的。三种培养基分别是：ＭＳ＋ＫＴＯ·ｌｍｇ／ｌ＋糖２０９／１＋琼脂７ｇ／ｌ、ＭＳ＋６—ＢＡ５ｍｇ／ｌ＋ＮＡＡＯ．０１ｍｇ／ｌ＋糖２０９／ｌ＋琼脂７ｇ／ｌ、１／ＺＭＳ＋ＮＡＡＯ．ｌｍｇ／ｌ＋糖２０ｇ／ｌ＋琼脂７ｇ／ｌ·三种培养基ｐＨ均为６。

A possible method to propagate Aster novi-beigli was obtained by tissue culture.The research showed:The proper propagating media were:(1) MS+KT 0.limg/1+sugar 20g/1+agar 7g/1(2) MS+6-BA 5mg/1+NAA 0.01mg/1+sugar 20g/1+agar 7g/1(3)1/2MS+NAA 0.1mg/1+sugar 20g/1+agar 7g/1The repid propagating aim was attained when the three media were use4 successively.