高活力纤维素酶菌株康氏木霉B—7的选育与产酶条件的研究

Study on the Selection of Trichoderma koningii B-7 With High Cellulase Actiity And the Conditions For Enzyme Production

野生型康氏木霉８５４－Ｂ＿２分别经物理化学诱变因子，ＴＤＰ辐射器和空间微重力辐射等因素的多级处理，得到１株形态发生明显改变的变异株Ｂ—７，其固体培养物的纤维素酶各组分酶活力，如滤纸糖酶活力（ＦＰＡ）为３４ｕ／ｇ，羧甲基纤维素酶活力（ＣＭＣ—ａｓｅ）为２９．０ｕ／ｇ，β－葡萄糖苷酶活力（β－Ｇｌａｓｅ）为２９．０ｕ／ｇ，与８５４－β２相比，分别提高５．９倍，７．６倍和４．２倍。其固体培养的最佳条件是：ｐＨ６．０，２８～３０℃，９６小时，最适培养基成分为：青草粉：麸皮＝７：３，１．５～２．０倍水和（ＮＨ４）２ＳＯ４１．５～２．０％（均以固体料计）。

A mutant B-7 with high cellulase activity was obtained from a wild type ofTrichoderma koningii 854-B2 by treatment of physical and chemical mutagens, microgravityand cosimic radiation. In semisolid sulture, the optimum conditionsfor enzyme productionare 7 portions of grass hay meal, 3 portions of wheat bran, 1. 5-2. 0 times of water and 0.15-2. 0% of amrnonium sulfate, PH6. 0, ar 28-30℃ for 96 hours. Under those conditions,its enzyme activities were 34u/g on filter paper, 1472u/g on carboxymethylcellulose and29u/g on D-salicin. Cellulase activities of B-7 were about 5. 9, 7. 6, 4. 2 times than thoseof the parent strain 854-B2.