rhGM—CSF对人骨髓粒单祖细胞集落形成及U937细胞分化的影响

Effects of rhGM-CSF on Granulocyte-macrophage Progenitor Cell Colony and U_(937) Cell Differentiation

本文应用造血祖细胞体外培养技术研究了重组人粒细胞－巨噬细胞集落刺激因子（ｒｈＧＭ—ＣＳＦ）对正常人骨髓粒单祖细胞集落（ＣＦＵ—ＧＭ）形成的影响，结果表明ｒｈＧＭ—ＣＳＦ在体外能促进细胞集落的形成，此种效应在一定范围内呈剂量依赖关系，与ＬＥＵＣＯＭＡＸ各剂量组相比无显著性差异。采用ＮＢＴ还原试验和ＡＰＡＡＰ法观察了ｒｈＧＭ—ＣＳＦ对Ｕ９３７细胞分化的影响，结果显示ｒｈＧＭ—ＣＳＦ能抑制Ｕ９３７细胞的增殖，促进其分化，部分细胞具有ＮＢＴ还原能力，ＣＤ１１６阳性细胞数增加。

By using hematopoietic progenitor culture, effect of rhGM-CSF on bone marrow progenitor cell colony formation was assayed. The results showed that rhGM-CSF can inducenormal human bone marrow mononuclear cell to form colony of granulocyte and macrophage(CFU-GM) with concentration-dependent manner. This effect was nonsignificant different compared with LEUCOMAX (p>0. 05). With NBT reduction test and APAAP, rhGM-GSF was evaluated for its capacity to influence the differentiation of U937 cell. After 3 daysculture in the presence of rhGM-CSF, a small but significant proportion of U937 cell wereable to increased CD11b expression.