RAPD鉴定栽培稻与野生稻体细胞杂种

Identification of Somatic Hybrids Between Rice Cultivar and Wild Oryza Species by RAPD

利用随机引物扩增DNA(RAPD)技术,对栽培稻和野生稻原生质体融合获得的体细胞杂种进行了鉴定。证实了它们包含有双亲的基因组成分,但来自双亲的基因组成分并不是对等的。一些体细胞杂种含有一个亲本更多的基因组成分,而另一些相反。利用RAPD数据和聚类图讨论了体细胞杂种和双亲的亲缘关系。

The somatic hybrids between rice cultivar and wild oryza species were obtained by PEG and electrical fusion method. The somatic hybrids were identified with RAPD. It was verified that the genome of somatic hybrids contained section genome of both parents ,but they were not equivalent one with the another. In the some somatic hybrids, the section genome from one parent was more than that from anohter. The relationship between somatic hybrids and their parents was analyzed based on RAPD data.