谷类作物半粒种子的PCR分析及其在标记辅助选择育种中的应用

PCR Analysis of Half-seeds of Cereal Crops and Its Application in Marker-assisted Selection and Breeding

建立了适于种子DNA分析的快速简便的PCR方法。水稻、小麦和玉米的半粒种子用提取缓冲液处理,产生的提取液可用于PCR和RAPD分析。水稻丰粒种子的DNA和来自叶片的DNA用专一的PCR引物扩增后可以产生同样的PCR产物。含有胚的另半粒种子可以正常发芽。将半粒种子的PCR分析方法用于水稻白叶枯病抗性基因型的鉴定,证明是植物育种和遗传学研究中一种非常实用的方法。

A simple and rapid PCR method were established in analyzing DNAs of seeds. Half-seeds of rice, wheat and maize were treated with an extraction buffer. The resulting supernatants were used in PCR and RAPD reactions. Identical PCR products were amplified with specific primers using either the half-seed DNA extracts or leaf tissue DNA extracts as templets. The reminant half-seeds with embryo could germinated nomally. PCR analysis of half-seeds were used in identifcation of bacterial blight resistance of rice and proved to be useful in plant breeding and genetics studies.