昆虫细胞(Sf9)培养中葡萄糖、乳酸的影响

Effects of Glucose and Lactic Acid on Insect Cell (Sf9) Culture

昆虫细胞培养已经在小儿灰髓炎、乙肝表面抗原等研究中得到系统应用,此外,许多高值的治疗性蛋白药物,如tPA、白细胞介素-2、β-干扰素和促红细胞生成素等的研究过程中昆虫细胞及杆状病毒的培养已成为新的生物工程手段。在美国、荷兰等国已成功地得到了外源基因大量表达的药物。在我国也已成功构建了人-α干扰素的昆虫细胞/杆状病毒表达系统。同时昆虫杆状病毒本身可以制备成各种专一性较强的广谱、无公害、无毒性的的农用杀虫剂。

Studied on the effects of the concentration of glucose and lactic acid on the cells growth in an insect cell (Sf9) culture. Adding different concentration of glucose to the medium TC-100, it has been determined that high glucose concentration (over than 2200mg/L) restrains cell growth. Analysing the concentration of lactic acid, it is shown that following culture time the concentration of lactic acid is decreased in the medium with PCS and increased in the serum-free medium. When the cell density is reached the maximun value, the concentration of lactic acid is to be decreased the minimum value. During the process of cell growth, the glucose is not transfomed to lactic acid, it enters TCA cycle or other metabolism paths. Cell culture conditions are temperature 27C, pH6. 2-6. 8 and cell inoculation density 2.0x105/rnl. While culture time is 96 hours, the maximum cell density, 2.3x106/ml, can be obtained.