摘要
目的:探讨宫颈癌细胞照射后存活后代生长特性及放射敏感性变化。方法:小鼠宫颈癌U14和其照射后存活后代体外培养,测定其群体倍增时间、放射敏感性和p53的表达水平。结果:U14群体倍增时间(92.0±4.8)h,克隆形成率为(36.0±0.8)%,SF2为0.45±0.06。U14照射后存活后代群体倍增时间为(126.4±8.6)h(t=4.763,P<0.05),克隆形成率为(15.0±1.5)%(t=9.647,P<0.01),SF2为0.84±0.04(t=4.625,P<0.05),p53表达水平无差别(t=0.846,P>0.05)。结论:宫颈癌细胞照射后存活后代生长延缓,放射敏感性降低,但与p53的表达无关。
Objective ：To investigate the change of radiosensitivity and gowth features of surviving progeny from irradiated cervicarcinoma cells. Methods：To cultured mice cervical carcinoma cells U14 and the progeny of irradicatecl cells U14, Plating efficency （PE） , Population doubling time（PDT） , radiosensitivity index SF2 and the expression level of p.53 were measured. Results：The PDT ofU14was （92.0±4.8）hrs, PE（36.0±0.8） % , SF2 （0.45±0.06）. The PDToftbe fitialcells was （126.4±8.6）hrs（t= 4. 763, P 〈 0. 05 ）, PE （ 15.0 ±1.5 ） % （ t = 9. 647, P 〈 0. 01 ）, SF2 （0. 84± 0. 04 ） （ t = 4. 625, P 〈 0. 05 ）. There was no statistical difference in the expression level of p53 between U14 and its progeny（ t = 0. 846, P 〉 0. 05 ）. Conclusion：In the present study, growth delay and declined radiosensitivity are confirmed in the progeny of irradiated cervical carcinoma cells, while those changes axe not correlated to the p53 expression.
出处
《临床肿瘤学杂志》
CAS
2007年第2期85-87,共3页
Chinese Clinical Oncology
基金
江西省卫生厅科技计划基金项目(0303087)
