TGFβ_1在子宫内膜样腺癌中的表达及其与DNA倍体相关性研究

Study on the expression of TGFβ_1 in the endometrioid adenocarcinoma and the relationship with DNA ploidy

目的:通过子宫内膜样腺癌DNA倍体分析,结合转化生长因子β1(TGFβ1)在子宫内膜样腺癌中表达的检测,从肿瘤演进和异质化的角度进一步探讨子宫内膜样腺癌侵袭与转移的分子生物学机制及其遗传学机制。方法:(1)采用免疫组化SP法对47例子宫内膜样腺癌、10例正常子宫内膜共计57例石蜡组织进行检测,所得结果用χ2检验进行比较分析;(2)采用流式细胞术对47例子宫内膜样腺癌石蜡组织进行DNA倍体检测。结果:(1)TGFβ1在子宫内膜样腺癌中的阳性表达率(78.7%)显著高于正常子宫内膜(30%)(P<0.01),TGFβ1表达强度与子宫内膜样腺癌临床分期、肌层浸润及淋巴结转移有关。(2)流式细胞术DNA异倍体率与TGFβ1阳性表达率有相关性。结论:肿瘤的演进和异质化是个复杂的过程,而恶变的瘤细胞分泌的TGFβ1及DNA异倍体共同反映了肿瘤的演进和异质化过程。联合检测上述指标有助于子宫内膜样腺癌患者的治疗和预后监测。

Objective：This study aimed to investigate DNA ploidy and the expression of TGFβ1 in the endometrioid adenocarcinoma was for the view of neoplasm heterogeneity, to explore the machinery of molecular biology and genetics for the endometrioid aclenocarcinoma. Methods： By immunohistechemical SP methods, we examined 47 cases of endometrioid adenocarcinoma, 10 cases normal endometrium, The results was treated by statistics methods -chi-square test. Flow cytometry DNA ploidy analysis was performed on 47 cases＇ of endometrioid adenocarcinoma, 10 cases of normal endometrium tissue with the use of paraffin-embeded sections. Results：The positive rate （78. 7% ） of TGFβ1 in the endometrioid adenocarcinoma was higher than that（ 30. 0% ） of normal endometrium. There was a positive relationship of TGFβ1 expression with muscles invasion depth, clinical stage and lymph node metastasis. There was a positive relationship of DNA ploidy with the expression of TGFβ1. Conclusion：It is a complex program for tumor progression and heterogeneity, and TGFβ1 DNA ploidy participate in the program together.