摘要
本文参照近缘物种的线粒体基因组序列,设计17对特异引物,采用LD-PCR、PCR及测序技术获得了我国广西产缅甸陆龟的线粒体全基因组序列,分析了其基因组特点和各基因的定位。结果表明:缅甸陆龟线粒体基因组全长为16813bp,碱基组成为35.30%A、26.47%T、12.09%G、26.14%C,包括13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码基因控制区(D-Loop区)。缅甸陆龟线粒体基因组各基因长度、位置与典型的脊椎动物相似,其编码蛋白质区域和rRNA基因与其它脊椎动物具有很高的同源性,显示龟类线粒体基因组在进化上十分保守。将缅甸陆龟的线粒体基因组序列提交到GenBank,获得的检索号为DQ656607。本文还结合GenBank中已发表的其它16种龟鳖类动物的线粒体基因组序列,探讨龟鳖类动物不同科间的系统进化关系。
We obtained the complete mitochondrial (mt) genome of lndotestudo elongata from individuals obtained in Guangxi, China. The full sequence was acquired from 17 LD-PCR and PCR-amplified fragments. The genome is very similar to other vertebrate mitochondrial genomes, containing 16 813 base pairs and encodes 37 genes, including 13 protein-coding, 2 ribosomal RNA and 22 transfer RNA genes. The base composition for the four nucleotides is 35.30% A, 26.47% T, 26.14% C and 12.09% G. The sequence of the rRNA genes, some of the protein-coding regions and the tRNA genes are highly homologous to those of other vertebrates. The sequence has been deposited in the GenBank database under the accession number DQ656607. In addition, we discuss the phylogenetic relationship among 16 tortoise and turtle species for which complete mitochondrial genome sequences are available in the public databases.
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2007年第1期151-158,共8页
ACTA ZOOLOGICA SINICA
基金
安徽省教育厅自然科学重点项目(2006kj113zd)
安徽省科学与技术后备人选基金项目(2006-2)
"生物环境与生态安全"安徽省高校重点室验室基金(2006)~~
关键词
缅甸陆龟
线粒体基因组
系统分析
Indotestudo elongata, Mitochondrial genome, Phylogenetic analysis